Designing multilayer perceptrons from nearest-neighbor systems

Although multilayer perceptrons have been shown to be adept at providing good solutions to many problems, they have a major drawback in the very large amount of time needed for training (for example, on the order of CPU days for some of the author's experiments). The paper describes a method of producing a reasonable starting point by using a nearest-neighbor classifier. The method is further expanded to provide a method of ;programming' the upper layer of any network assuming the lower layers already exist.     

Cocontraction changes in muscular fatigue at different levels of isometric contraction

Ten normal subjects participated in a study designed to contrast results obtained in pre- and post-fatigue states. The measures contrasted were the IEMG ratios of agonist/antagonist pairs of muscles. The experimental task was an 8 s ramp isometric elbow flexion ranging from 0 to 100% of a maximal voluntary contraction (MVC). IEMG ratios were obtained at levels of 20, 40, 60 and 80% MVC. Records from the following muscles were obtained with surface electrodes: biceps brachii (BB), brachioradialis (BR), triceps brachii (TB) and anconeus (SU). The torque at the elbow joint was measured by a Cybex II dynamometer. Fatigue was induced using a 60% MVC of elbow flexion maintained during 5 minutes. The data were collected on-line at a sampling rate of 1 kHz. The results indicated that the IEMG ratios (BB/TB and BR/SU) presented a tendency, across subjects, toward a decrease at the levels of 40%, 60% and 80% MVC at the post- relative to the pre-fatigue state. The BB/BR ratios remained stable. These changes in the post-fatigue ratios disclose a tendency toward a saturation of the agonist occurring concomitantly with an increased level of contraction of the antagonists.     

Cell cycle effects of the novel topoisomerase I inhibitor NU/ICRF 505 in a panel of Chinese hamster ovary cell lines

The effect of the novel topoisomerase I inhibitor NU/ICRF 505 (20 microM, approximate IC50 concentration) on the cell cycle was studied by flow cytometry in four Chinese hamster ovary (CHO) cell lines. Postdrug treated cells were incubated with optimal concentrations of cytochalasin B to prevent re-entry of daughter cells into the cell cycle. NU/ICRF 505 had no significant effect on cell cycle distribution in the parent cell line (CHO-K1) and two mutants hypersensitive to topo II inhibitors (ADR-1, ADR-3), all of which express similar levels of topo I protein. In the drug-resistant variant ADR-r, which overexpresses topo I 2-fold, a significant accumulation of cells in G1 phase was recorded. These results are broadly consistent with the cell cycle effects expected in CHO cells by a classic topo I poison (camptothecin) and add weight to the view that NU/ICRF 505 induces cell death primarily through topo I inhibition.     

Endobronchial tumours in childhood

Like psychomotor stimulants, a weak amphetamine-like agent, such as phenylpropanolamine, enhances the analgesic effects of morphine (MOR). Thus, it is possible that full psychomotor stimulant potency is not required to increase the analgesic action of opiates. The validity of this assumption is here tested by studying the ability of (-)-norpseudoephedrine (NPE), an enantiomer of phenylpropanolamine and a metabolite of cathinone, to influence both the analgesic effects of MOR and its discriminative stimulus properties. In mice NPE (5.6-10.0-17.0 mg/kg i.p.) did not prolong the latency to lick or to remove paws from a plate warmed at 54 degrees C. However, it significantly potentiated the analgesic effect of 3.2 mg/kg of MOR. These results were replicated in rats by use of the formalin test, which measures the numbers of hind paw flinches produced by injecting 50 microl of formalin into the dorsal surface of the paw. The higher dose of NPE (17 mg/kg) increased the effect of sub-analgesic doses of MOR (0.56 and 1.0 mg/kg). In rats trained to discriminate between 0.5 mg/kg of amphetamine and solvent in a two-lever operant behavior reinforced by water access. NPE induced a dose-dependent increment of drug lever responding from 0% at 1.0 mg/kg to 100% at 32.0 mg/kg. In contrast, NPE did not generalize for the MOR cue up to the dose of 56.0 mg/kg, which produced a substantial reduction of the response rate. However, when given in combination, NPE attenuated the discriminative effects of MOR and potentiated its inhibitory action on the response rate. These results exclude a direct action of NPE on the mu opiate system. In conclusion, NPE preserves amphetamine-like properties and these properties are probably responsible for the interaction of the drug with the analgesic and discriminative effects of MOR. Therefore, this study contradicts the assumption that the analgesic effects of MOR can be enhanced by a sympathomimetic drug that lacks significant psychostimulant actions.     

Energy storage in ceramic dielectrics

An evaluation has been made of the energy storage capabilities of ceramic dielectrics that were considered likely to provide high energy/volume efficiency on the basis of their expected permittivity-field characteristics. Data for fields up to 400 kV/cm are presented for a strontium titanate, and for a barium titanate ceramic. The materials were in thick-film form and bonded with a small amount of glass. At the maximum fields, energy storage in the barium titanate ceramic was close to that reported earlier for glass-bonded lead zirconate (approx. 2.0 J/cm³), but was about 30% lower in the strontium titanate material.     

Temperature-regulated expression of the tac/lacl system for overproduction of a fungal xylanase in Escherichia coli

Temperature-regulated expression of recombinant proteins in the tac promoter (Ptac) system was investigated. Expression levels of fungal xylanase and cellulase from N. patriciarum in E. coli strains containing the natural lacI gene under the control of the Ptac markedly increased with increasing cultivation temperature in the absence of a chemical inducer. The specific activities (units per milligram protein of crude enzyme) of the fungal xylanase and cellulase produced from recombinant E. coli strain pop2136 grown at 42 degrees C were about 4.5 times higher than those of the cells grown at 23 degrees C and were even slightly higher when compared with cells grown in the presence of the inducer isopropyl beta-D-thiogalactopyranoside. The xylanase expression level in the temperature-regulated Ptac system was about 35% of total cellular protein. However, this system can not be applied to E. coli strains containing lacIq, which confers over production of the lac repressor, for high-level expression of recombinant proteins. In comparison with the lambda PL system, the Ptac-based xylanase plasmid in E. coli pop2136 gave a considerably higher specific activity of the xylanase than did the best lambda PL-based construct using the same thermal induction procedure. The high-level expression of the xylanase using the temperature-regulated Ptac system was also obtained in 10-litre fermentation studies using a fed-batch process. These results unambiguously demonstrated that the temperature-modulated Ptac system can be used for overproduction of some non-toxic recombinant proteins.     

North American Society of Pacing and Electrophysiology policy statement. The NASPE/BPEG defibrillator code

A new generic code, patterned after and compatible with the NASPE/BPEG Generic Pacemaker Code (NBG Code) was adopted by the NASPE Board of Trustees on January 23, 1993. It was developed by the NASPE Mode Code Committee, including members of the North American Society of Pacing and Electrophysiology (NASPE) and the British Pacing and Electrophysiology Group (BPEG). It is abbreviated as the NBD (for NASPE/BPEG Defibrillator) Code. It is intended for describing the capabilities and operation of implanted cardioverter defibrillators (ICDs) in conversation, record keeping, and device labeling, and incorporates four positions designating: (1) shock location; (2) antitachycardia pacing location; (3) means of tachycardia detection; and (4) antibradycardia pacing location. An additional Short Form, intended only for use in conversation, was defined as a concise means of distinguishing devices capable of shock alone, shock plus antibradycardia pacing, and shock plus antitachycardia and antibradycardia pacing.